All in-room measurements are affected by reflections, but there is a difference between short and long wavelengths.
For short wavelengths, e.g. a 20kHz sound has a 17.15mm wavelength (about 2/3"), several full cycles will be produced by the speaker before it reflects and arrives at the measurement mic. Thus it can be easily windowed out.
As wavelengths get longer, we reach a point where a single wavelength reflects before it reaches the mic. Measurement of any frequencies below this do not give the speaker response, but the room + speaker response. We can calculate this lower frequency limit with this equation:
F =
c / (2* (x^2 + d^2)^0.5)
Where c = speed of sound (343m/s or 1125ft/s), x = 1/2 the distance between microphone and speaker, and d = distance to the nearest reflecting surface, usually the floor.
Source.
Thus for a mic placed 1m from the speaker and 1m from the floor, we have 343 / (2*0.5^2 + 1^2)^0.5) = 153Hz. If you have a sofa which is 30cm from the microphone, it is 294Hz, meaning any frequency measured <294Hz is nonsense.
Bear in mind that microphone stands can be reflecting surfaces and contribute to little wiggles in the FR. Read more about it
here.
Note this is
not the Schroder frequency, that is something else entirely. Of course, the measurement is valid if our intention is room correction. But it is not a valid measurement of loudspeaker performance below a certain frequency.