Okay, the electronics background really helps. Otherwise I would've probably just dismissed you at this point, honestly. There's a ton to work on and VituixCAD is your friend. Honestly, starting from a sub-bass unit is a little weird, but it means you'll be working from that maximum SPL standpoint. So you'll have to calculate that. The box can be simulated in VCAD, including isobarics and multiple drivers in a single volumetric space. It can do a lot of ports and stuff.
You'll then have to find a midrange/midwoofer that can meet it, same with a tweeter. Matching dispersion and choosing a driver layout is hard and I suggest you google that. The front baffle will radiate and affect driver responses. Box design is a whole can of worms as a total volume in a formula vs six resonating panels. Essentially you need to tie each panel to each other in an effective way to quell resonance. Damping is a whole other bag of cats.
You should know more than I do already about crossovers if your electronics background is legit. 3-ways will have a ton of parts, a low-pass, a high-pass, a combo on the midrange, maybe some notches for leveling impedance... It can be a lot. Crossovers affect phase and will determine which parts of the driver's directivity applies to the speaker. This can include cancellations depending on the driver layout and your filter orders and your turbo encabulator. Getting dispersion to match and work (especially vertically) is the greatest challenge. The best distances (center-to-center) are 1/4-1/3 crossover wavelength. 6/5 is supposed to work as well.
A mic and some drivers are the best and most reliable way to build a speaker, and a multi-way DSP controller will definitely be the most effective turnkey solution, requiring no additional analog components in the system. A MiniDSP 4x8, Hypex FAxx3, or some competitor or equivalent will do wonders in an afternoon.
My last suggestion is to build the sub section in an opposed format, either cone-to-cone or magnet-to-magnet, so they can be run non-isobaric in a DOS format, or if the MFB is adequate for distortion control and extension. On the other hand, that Dayton seems designed to work with an available passive radiator, which can also be simulated in VituixCAD with a quick copy/paste from the loudspeakerdatabase.com and will similarly afford a lower fs without all the drawbacks of isobarics.
I'm sure the folks here and the friendly people at DIYAudio will be happy to help with specific questions.