Key point here, once again: If there is a difference detected in 1) so that we may safely assume the signal has actually changed then this can more or less easily be measured and the difference in the signal exposed. It is important that the complete setup must not change between listening test and measurements, otherwise one might introduce unknown variables. A preamp in a complete system may behave very different (in what exacly the power amp sees at its inputs) than when tested on the bench in isolation, for example.
The harder part is to correlate what changes seen in the difference are actually dominant for the perception change but with experience this can be mastered and control tests can be made that probe the hypothesis.
The sad part, in my experience so far, is that the Golden Ears have always refused to take part in such a test (I've offered my technical assistance to such efforts, but to no avail), even when discussed privately with the option that if 1) fails, the whole incident would be declared as "it never happened" (and would not be published) so nobody would loose their face.
As what what miniscule differences in audio signals can actually be isolated, take a look here:
https://www.audiosciencereview.com/...dible-with-music-signals-some-examples.20886/
This is dealing with stuff that is many orders of magnitude below any reasonable thresholds of hearing... and this makes me very confident to put forth the bold statement that really everything that can heard in controlled listening tests always can be measured/identified. No exceptions.