Paper, cotton, textile,soft, hard, aluminum, gold, beryllium, plastic, ribbon, nylon, silk, silver, ceramic, kevlar, glue, stitches, geometry, dots, lines, tweeter's wave guide, rubber, felt, metal, foam, fiberglass, ...they all have various tonal characteristics.
The spider, the voice coil, the magnet material, the diameter, the length, the structure, the composition of the drivers, the crossover parts, their slopes,the frequencies where drivers cross, their united designs, the box enclosures, woods, density, thickness, the bracing, the material used inside the box, the glue, the aluminum rods, the rigidity, the non-parallel walls, the curvatures, the phase tuning, the positioning of the drivers, their isolation and insulation from internal interference, the external shape, the 360° polar dispersion, the overall internal and external design with all the parts selected and computer tuned, the part's values, their selection, the research, the experiments, the wiring, the circuit boards (x-overs), ...all contribute to the speaker's final "identity/tonality".
Burn-in time? That too. Colors? No.
...Only psychologically.